IFN beta provides immuno protection in the retina by inhibiting ICAM 1 and cxcl9 in retinal pigment epithelial cells. (2008 Mar)
IFN beta provides immuno protection in the retina by inhibiting ICAM 1 and cxcl9 in retinal pigment epithelial cells . The retinal pigment epithelial ( RPE ) cell is a potent regulatory cell that facilitates normal physiologic processes and plays a critical role in a variety of retinal diseases . We evaluated IFN beta production in human RPE cells through TLR signaling and investigated the effects of IFN beta on RPE cells . RPE cells treated with poly ( I : C ) or infected with an RNA virus produce IFN beta . kinetic studies revealed that IFN beta levels continue to increase over a 48 h period and this was associated with the up regulation of IRF 7 gene expression , a known positive feedback molecule for IFN beta production . microarray analysis revealed that in IFN beta treated cells , 480 genes of 22 , 283 genes were up or down regulated by 2 fold . We hypothesize that IFN beta induction during TLR signaling in the retina is an immunosuppressive factor produced to limit immunopathologic damage . cytokine activation of RPE cells results in the production of the chemokines , cxcl9 and cxcl10 , and the adhesion molecule , ICAM 1 . pretreatment of RPE cells with IFN beta resulted in inhibition of ICAM 1 production and elimination of cxcl9 production . This treatment did not alter cxcl10 production . Anti IFN beta Ab blocked the inhibitory action of IFN beta . Real time PCR analysis revealed …

Endothelial adhesion molecules and their role in inflammation. (1993 Jul)
endothelial adhesion molecules and their role in inflammation . The emigration of leukocytes such as neutrophils into inflammatory sites requires adhesion to the endothelium of small venules . The initial adhesive event is margination characterized by rolling of neutrophils along the luminal surface of the endothelium . Each member of the selectin family of adhesion molecules has been shown to support neutrophil rolling under conditions of flow . E selectin is synthesized by endothelial cells following cytokine stimulation , P selectin is rapidly mobilized from weibel palade bodies to the endothelial cell surface following stimulation with agents such as histamine , and L selectin is constitutively expressed on the surface of leukocytes . Each selectin functions primarily as a lectin , recognizing carbohydrate structures on the leukocyte or endothelial cell surface . Once the marginated neutrophil forms a stationary adhesion with endothelial cells , it is stimulated by chemotactic factors to downregulate the selectin based adhesion and upregulate adherence dependent on beta 2 integrins , principally cd11a / CD18 ( LFA 1 ) and cd11b / CD18 ( Mac 1 ) . these adhesion molecules interact with intercellular adhesion molecule 1 ( ICAM 1 ) and possibly other structures on the endothelial cell , and the leukocyte rapidly emigrates into surrounding tissue . transendothelial migration in vitro is markedly inhibited by monoclonal antibodies against CD18 integrins or ICAM 1 . monoclonal antibodies against the selectins , CD18 , cd11a , cd11b , and ICAM 1 have all been shown to …

Role of intra cellular adhesion molecule 1 ( ICAM 1 ) and its soluble form ( sicam ) in chronic … (2004 Jun)
Role of intra cellular adhesion molecule 1 ( ICAM 1 ) and its soluble form ( sicam ) in chronic airway inflammation mucosal inflammation is the feature of both bronchial asthma and allergic rhinitis with evident of tissue eosinophilia , mast cells , eosinophils and T lymphocytes activation . The initial phase of cell recruitment is the margination and adhesion of leucocytes to the endothelium , prior to their transendothelial migration under a directed chemotactic stimulus . This adhesion occurs through specific ligand receptor couplets involving leucocyte endothelial adhesion molecules . One of these cell adhesion molecules is ICAM 1 , an important early marker of immune activation and response . Its ligand , leukocyte function associated antigen one ( LFA 1 ) is expressed on neutrophils , eosinophils and T cells . ICAM 1 was found to be expressed on epithelial and endothelial cells in rhinitis patients and in bronchial biopsies obtained from asthmatics also after allergen challenge . circulating forms of these adhesion molecules have been identified in the peripheral blood , bronchoalveolar lavage ( BAL ) fluid and nasal lavage in patients with asthma and rhinitis . systemic and local up regulation of sicam 1 suggests a function role for this soluble form of ICAM in the allergic inflammation .

Human / severe combined immunodeficient mouse chimeras. (1993 Apr)
human / severe combined immunodeficient mouse chimeras . An experimental in vivo model system to study the regulation of human endothelial cell leukocyte adhesion molecules . The ability of circulating white blood cells to enter inflamed tissues is mediated by specific cell adhesion molecules thought to be expressed in a programmed and sequential manner to form an adhesion cascade . because of the complexity of this process , it is becoming increasingly important to develop in vivo models . Two major problems have limited the utility of current animal models . The first is the inability of many of the antibodies developed against cell adhesion molecules in human cell culture models to cross react in animals . The second is the uncertainty in extrapolating animal ( particularly rodent ) findings to humans . To circumvent these problems , full thickness human skin grafts were transplanted onto immunodeficient ( severe combined immunodeficient ) mice . after 4 6 wk , the transplanted skin grafts closely resembled normal skin histologically and maintained their human vasculature as determined by immunohistochemical staining with human specific endothelial cell markers . intradermal injection of tumor necrosis factor alpha resulted in the reversible upregulation of the leukocyte endothelial adhesion molecules E selectin , vascular cell adhesion molecule 1 , and intercellular adhesion molecule 1 , and in an active inflammatory reaction with migration of murine leukocytes into cytokine injected areas . these results indicate that the severe combined immunodeficient mouse / human skin transplant model provides a …

Expression of cell adhesion molecules in the lungs of patients with idiopathic pulmonary fibrosis. (1995 Aug)
expression of cell adhesion molecules in the lungs of patients with idiopathic pulmonary fibrosis . idiopathic pulmonary fibrosis ( IPF ) is a chronic inflammatory disorder restricted to the lungs . leukocyte entry into the area of inflammation is regulated , at least partly , by endothelial expression of leukocyte selective cell adhesion molecules ( CAMs ) . To investigate the relevance of these CAMs to the accumulation of leukocytes in IPF , we examined the expression of E selectin ( endothelial leukocyte adhesion molecule 1 ; ELAM 1 ) , intercellular adhesion molecule 1 ( ICAM 1 ) , and vascular cell adhesion molecule 1 ( VCAM 1 ) by immunohistochemistry in lung tissue from nine patients with IPF and five nonsmoking normal subjects . The results demonstrated that in normal lungs , ICAM 1 was weakly expressed on endothelial cells , but neither E selectin nor VCAM 1 was detected . In the lungs of patients with IPF , E selectin expression on endothelial cells was restricted to honeycombing regions . endothelial expression of ICAM 1 was increased throughout the tissue , but VCAM 1 was not detected in IPF . The distribution of leukocytes in lungs with IPF consisted of mostly lymphocyte accumulation in the interstitium and neutrophil accumulation within the airspaces of honeycomb regions . these results suggest that E selectin may play a role in the recruitment of neutrophils in regions of honeycombing and that ICAM 1 may play a role in lymphocyte recruitment into …

Host defenses against disseminated candidiasis are impaired in intercellular adhesion molecule 1 deficient mice. (1996 Sep)
Host defenses against disseminated candidiasis are impaired in intercellular adhesion molecule 1 deficient mice . genetically engineered mice , which lack normal expression of intercellular adhesion molecule 1 ( ICAM 1 ) , were used to study the role of ICAM 1 in the host defense against disseminated candidiasis . The responses of ICAM 1 deficient mice and normal wild type mice were compared following an intravenous challenge with candida albicans . ICAM 1 deficient mice lost more weight ( P . 001 ) and had a significantly higher mortality ( P . 001 ) . quantitative cultures revealed a greater tissue fungal burden in ICAM 1 deficient mice compared with normal mice , in both the kidney ( P . 001 ) and the brain ( P . 007 ) . extensive inflammation , composed primarily of histiocytes admixed with lymphocytes and occasional neutrophils , was present in the renal tissue of ICAM 1 deficient mice ; this contrasted with a more localized and predominantly neutrophilic infiltrate in normal mice . This work suggests that the loss of ICAM 1 significantly impairs host defense against C . albicans , by impairing either neutrophil migration or phagocyte activation or both .

Cross talk between ICAM 1 and granulocyte macrophage colony stimulating factor receptor signaling modulates eosinophil survival and activation. (2008 Mar)
cross talk between ICAM 1 and granulocyte macrophage colony stimulating factor receptor signaling modulates eosinophil survival and activation . reversal of eosinophilic inflammation has been an elusive therapeutic goal in the management of asthma pathogenesis . In this regard , GM CSF is a primary candidate cytokine regulating eosinophil activation and survival in the lung ; however , its molecular mechanism of propagation and maintenance of stimulated eosinophil activation is not well understood . In this study , we elucidate those late interactions occurring between the GM CSF receptor and activated eosinophil signaling molecules . using coimmunoprecipitation with GM CSF stimulated eosinophils , we have identified that the GM CSF receptor beta chain ( gmrbeta ) interacted with ICAM 1 and Shp2 phosphatase , as well as slp76 and ADAP adaptor proteins . separate experiments using affinity binding with a tyrosine phosphorylated peptide containing an ITIM ( ICAM 1 residues 480 488 ) showed binding to Shp2 phosphatase and gmrbeta . however , the interaction of gmrbeta with the phosphorylated ICAM 1 derived peptide was observed only with stimulated eosinophil lysates , suggesting that the interaction of gmrbeta with ICAM 1 required phosphorylated Shp2 and / or phosphorylated gmrbeta . importantly , we found that inhibition of ICAM 1 in activated eosinophils blocked GM CSF induced expression of c fos , c myc , IL 8 , and TNF alpha . moreover , inhibition of ICAM 1 expression with either antisense oligonucleotide or an ICAM 1 blocking Ab effectively inhibited …

Expression of the adhesion molecules ICAM 1 , VCAM 1 , LFA 1 and VLA 4 in the skin is … (1999 Apr)
expression of the adhesion molecules ICAM 1 , VCAM 1 , LFA 1 and VLA 4 in the skin is modulated in progressing stages of chronic venous insufficiency . In inflammation and wound healing , dynamic changes in cell adhesion and migration are fundamental properties of the cells involved . disturbed interaction of leukocytes with microvascular endothelial cells has been proposed to be a central pathogenic factor in chronic venous insufficiency . This disease may therefore serve to elucidate dysregulated modulation of adhesion molecule expression in conditions of chronic inflammation and impaired wound healing . In this study , we determined how the expression of ICAM 1 / VCAM 1 on endothelial cells and their ligands LFA 1 / VLA 4 on leukocytes is modulated in skin of progressing stages of chronic venous insufficiency . immunohistochemical staining of skin biopsies revealed an increase in the expression of ICAM 1 and VCAM 1 on endothelial cells in an early stage of venous disease such as stasis dermatitis . Such protein expression correlated with an increase of corresponding mRNA in skin biopsies . expression of these CAMs on endothelial cells was accompanied by the occurrence of a marked perivascular infiltration of leukocytes , which expressed increased levels of LFA 1 and VLA 4 . In progressing stages of chronic venous insufficiency , characterized by hyperpigmentation and lipodermatosclerosis , which precede skin ulceration , all these CAMs remained upregulated on endothelial cells and infiltrating leukocytes . Our findings indicate that following an initial …

4 O methylgallic acid down regulates endothelial adhesion molecule expression by inhibiting NF kappab DNA binding activity. (2006 Nov)
4 O methylgallic acid down regulates endothelial adhesion molecule expression by inhibiting NF kappab DNA binding activity . We here investigated the functional effect of 4 O methylgallic acid ( 4 OMGA ) , a major metabolite of gallic acid abundant in red wine , on vascular inflammation and its action mechanism . 4 OMGA inhibited the expression of intercellular adhesion molecule 1 ( ICAM 1 ) and vascular cell adhesion molecule 1 ( VCAM 1 ) in human umbilical vein endothelial cells ( huvecs ) stimulated with tumor necrosis factor alpha ( TNF alpha ) , resulting in the suppression of leukocyte adhesion to huvecs . In addition , 4 OMGA inhibited the promoter activities of ICAM 1 and VCAM 1 and the activity of nuclear factor kappab ( NF kappab ) without affecting cytosolic ikappab kinase ( IKK ) activation , inhibitor of kappab ( ikappab ) phosphorylation and degradation , and nuclear translocation of NF kappab . This compound did not alter nitric oxide ( NO ) generation , but inhibited reactive oxygen species ( ROS ) production in TNF alpha stimulated huvecs , suggesting that NO and ROS are not involved in 4 OMGA mediated inhibition of NF kappab activity . moreover , 4 OMGA directly blocked the binding activity of NF kappab to its consensus DNA oligonucleotide , when pre incubated with the nuclear extract from TNF alpha stimulated huvecs , but not with the oligonucleotide alone . This inhibition was completely abolished by the …

Pathologic roles of prorenin and ( pro ) renin receptor in the eye. (2008 May)
pathologic roles of prorenin and ( pro ) renin receptor in the eye . recent reports indicated that tissue renin angiotensin system ( RAS ) was upregulated and angiotensin II type 1 receptor signaling plays crucial roles in ocular inflammation and neovascularization ; however , the precise mechanism for activating tissue RAS had not been defined until recently . ( Pro ) renin receptor , a recently identified molecule existing in the major organs but not in the circulation , has attracted growing attention as an activator of tissue RAS . When the handle region of the prorenin prosegment binds to ( pro ) renin receptor , prorenin undergoes a conformational change to its enzymatically active state without the conventional proteolysis of the prorenin prosegment . systemic treatment with a peptide with the structure of the handle region ( handle region peptide ; HRP ) , which competitively binds to ( pro ) renin receptor as a decoy peptide and inhibit the nonproteolytic activation of prorenin , resulted in the suppression of retinal inflammation and neovascularizaion in the rodent models . retinal expression of RAS related inflammatory and angiogenic molecules , such as intercellular adhesion molecule 1 , monocyte chemotactic protein 1 , and vascular endothelial growth factor , was also suppressed with application of HRP . these findings demonstrate that nonproteolytically activated prorenin plays a significant role in the ocular inflammation and neovascularization .

Direct contact between human peripheral blood mononuclear cells and renal fibroblasts facilitates the expression of monocyte chemoattractant protein 1. (2003 Jul)
direct contact between human peripheral blood mononuclear cells and renal fibroblasts facilitates the expression of monocyte chemoattractant protein 1 . background : Cell to cell interaction is thought to be an important feature of a variety of biological processes . As far as the proinflammatory process is concerned , the interaction between mesangial cells and monocytes / macrophages induces the expression of monocyte chemoattractant protein 1 ( MCP 1 ) , and this may play a role in glomerulonephritis . In this study , we investigated whether the cell to cell interaction between immune cells and renal fibroblasts induces MCP 1 gene expression , which may be involved in interstitial inflammation in the kidney . methods : human renal fibroblast cell lines , tNKF ( from a normal kidney ) and tfkif ( from a kidney with fibrosis ) , and peripheral blood mononuclear cells ( PBMC ) were used to assess the effect of cell to cell contact on the expression of MCP 1 mRNA in the fibroblasts . The expression of the MCP 1 gene in the fibroblasts was also examined after stimulation with tumor necrosis factor alpha ( TNF alpha ) and the culture supernatant from PBMC . RT PCR was used to detect MCP 1 mRNA expression . neutralizing antibodies to intercellular adhesion molecule 1 ( ICAM 1 ) and vascular endothelial adhesion molecule 1 ( VCAM 1 ) were used to block the cell to cell contact between the fibroblasts and PBMC . results : …

Clinical and immunologic effects of a rush sublingual immunotherapy to parietaria species : A double blind , placebo controlled trial. (1999 Dec)
clinical and immunologic effects of a rush sublingual immunotherapy to parietaria species : A double blind , placebo controlled trial . background : The local ( noninjection ) routes of immunotherapy are presently regarded as viable therapeutic options for respiratory allergy , and their mechanisms of action are currently undergoing investigation . objective : We evaluated the clinical efficacy of a preseasonal rush sublingual swallow immunotherapy and its effects on allergic inflammation in patients with seasonal rhinoconjunctivitis caused by parietaria species . methods : thirty patients with parietaria species induced rhinoconjunctivitis ( 13 with mild intermittent asthma ) were randomly assigned sublingual swallow immunotherapy or placebo in a rush preseasonal course . We assessed the seasonal symptom drug intake score by diary card and the inflammatory infiltration and the intercellular adhesion molecule 1 expression on nasal epithelium after specific allergenic challenge before and after treatment . results : The investigated immunotherapy was well tolerated , and no side effects were recorded . A significant reduction of the symptom score ( P . 016 ) and drug intake score ( P . 008 ) after immunotherapy was observed only in the active group . A decrease of the cumulative score was observed also in the placebo group ( P . 046 ) , but the significance was clearly higher ( P . 006 ) in the active group . In the active group a reduction of neutrophils ( P . 001 ) , eosinophils ( P . 01 ) , and …

Hormone replacement therapy and circulating ICAM 1 in postmenopausal women a randomised controlled trial. (1999 Jul)
hormone replacement therapy and circulating ICAM 1 in postmenopausal women a randomised controlled trial . hormone replacement therapy may reduce the risk of coronary heart disease but underlying mechanism has not been adequately explained . recent data suggest that intercellular adhesion molecule 1 ( ICAM 1 ) plays a critical role in early stage of atherosclerosis and may serve as a molecular marker for the development of arterial disease . We investigated the effects of oral and transdermal cyclic oestradiol combined with progesterone on plasma concentration of soluble ICAM 1 ( sicam 1 ) . thirty seven healthy postmenopausal women were randomly assigned to receive either oral estradiol valerate or transdermal estradiol both combined with micronized progesterone or no hormonal treatment . plasma sicam 1 was assayed at baseline and after a 6 month period . Oral but not transdermal estradiol regimen significantly decreased mean value of sicam 1 compared with no treatment . differences in sicam 1 levels between active treatments were significant . there were no significant changes in mean values of fibrinogen between the three groups . Our results show a favorable effect of oral estrogen plus progesterone on a soluble marker of vascular inflammation and may provide plausible explanation for a cardioprotective effect of hormone replacement therapy among healthy postmenopausal women .

Plasma concentration of soluble intercellular adhesion molecule 1 and risks of future myocardial infarction in apparently healthy men. (1998 Feb)
plasma concentration of soluble intercellular adhesion molecule 1 and risks of future myocardial infarction in apparently healthy men . background : The intercellular adhesion molecule ICAM 1 mediates adhesion and transmigration of leucocytes to the vascular endothelial wall , a step proposed to be critical in the initiation and progression of atherosclerosis . whether concentrations of soluble ICAM 1 ( sicam 1 ) are raised in apparently healthy individuals who later suffer acute myocardial infarction is unknown . methods : We obtained baseline plasma samples from a prospective cohort of 14 , 916 healthy men enrolled in the physicians health study . With a nested case control design , we measured sicam 1 concentrations for 474 participants who developed a first myocardial infarction , and 474 controls ( participants who remained healthy throughout the 9 year follow up ) . cases were matched to controls according to age and smoking status at the time of myocardial infarction . findings : We found a significant association between increasing concentration of sicam 1 and risk of future myocardial infarction ( p 0 . 003 ) , especially among participants with baseline sicam 1 concentrations in the highest quartile ( 260 ng / mL ; relative risk 1 . 6 95 Cl 1 . 1 2 . 4 , p 0 . 02 ) . This association was present overall as well as among non smokers , and persisted after control for lipid and non lipid risk factors . In multivariate analyses , …

Circulating soluble adhesion molecules in patients with giant cell arteritis. (1999 Jun)
circulating soluble adhesion molecules in patients with giant cell arteritis . correlation between soluble intercellular adhesion molecule 1 ( sicam 1 ) concentrations and disease activity . objective : To evaluate whether changes in concentrations of circulating adhesion molecules are related to disease activity in patients with giant cell arteritis ( GCA ) . methods : A sandwich elisa was used to measure soluble intercellular adhesion molecule 1 ( sicam 1 ) , sicam 3 , vascular cell adhesion molecule 1 ( svcam 1 ) , E selectin ( sE selectin ) , and L selectin ( sL selectin ) in serum and plasma samples from patients with GCA . A cross sectional study was performed on 64 GCA patients at different activity stages and on 35 age and sex matched healthy donors . thirteen of these patients were evaluated at the time of diagnosis and serially during follow up . results : At the time of diagnosis , sicam 1 concentrations were significantly higher in active GCA patients than in controls ( mean ( SD ) 360 . 55 ( 129 . 78 ) ng / ml versus 243 . 25 ( 47 . 43 ) ng / ml , p 0 . 001 ) . In contrast , sicam 3 , svcam 1 , sE selectin , and sL selectin values did not differ from those obtained in normal donors . With corticosteroid administration , a decrease in sicam 1 concentrations was observed , reaching normal values when …

Hydrogen peroxide augments eosinophil adhesion via beta2 integrin. (2000 Dec)
hydrogen peroxide augments eosinophil adhesion via beta2 integrin . during eosinophil ( EOS ) accumulation at sites of allergic inflammation , an initial step is the binding of EOS to adhesion molecules expressed on vascular endothelial cells ( EC ) . We have previously observed that adhesion of peripheral blood EOS to recombinant human vascular cell adhesion molecule 1 ( rh VCAM 1 ) stimulates the respiratory burst of EOS . although the biological consequence of this activation remains to be elucidated , reactive oxygen species such as hydrogen peroxide ( H2O2 ) may modify the adhesive property of EOS . In the present study , we examined whether H2O2 modifies the adhesive property of EOS . EOS were isolated from the peripheral blood of healthy subjects . adhesion of the EOS to paraformaldehyde fixed human umbilical vein EC ( huvec ) , stimulated or not stimulated with tumour necrosis factor alpha ( TNF alpha ; 100 pM for 24 hr ) , was examined in the presence or absence of H2O2 . H2O2 significantly enhanced adhesion of EOS to both resting and TNF alpha stimulated fixed huvec ( P 0 . 01 , respectively ) . Such enhancing effects were inhibited by anti beta2 integrin antibody or anti cd11b antibody , but not by anti cd11a or anti alpha4 integrin antibody . H2O2 also enhanced EOS adhesion to rh intracellular cell adhesion molecule 1 ( ICAM 1 ) but not to rh VCAM 1 . finally , H2O2 enhanced …

Carotid atherosclerosis is associated with inflammation , malnutrition and intercellular adhesion molecule 1 in patients on continuous ambulatory peritoneal dialysis. (2004 Apr)
carotid atherosclerosis is associated with inflammation , malnutrition and intercellular adhesion molecule 1 in patients on continuous ambulatory peritoneal dialysis . background : recent evidence suggests that endothelial cell adhesion molecules may participate in the initiation and progression of atherosclerotic vascular damage . The aim of the present report was to investigate serum intercellular adhesion molecule 1 ( ICAM 1 ) , vascular cell adhesion molecule 1 ( VCAM 1 ) and E selectin concentrations and their probable association with atherosclerotic disease in patients on continuous ambulatory peritoneal dialysis ( CAPD ) . methods : sixty three CAPD patients and 40 age and sex matched apparently healthy normotensive controls participated in the study . atherosclerotic disease in both groups was assessed by measuring the intima media thickness ( IMT ) and plaque score of the common carotid arteries using an ultrasound scanner . results : compared with controls , CAPD patients had significantly increased IMT and plaque score values ( P 0 . 001 and P 0 . 0001 , respectively ) , as well as serum ICAM 1 , VCAM 1 and E selectin concentrations ( P 0 . 0001 , P 0 . 0001 and P 0 . 05 , respectively ) . In univariate analyses , IMT values were significantly correlated with age , systolic blood pressure ( BP ) , logcrp , fibrinogen , albumin and ICAM 1 levels ( P 0 . 001 , P 0 . 04 , P 0 . 01 , P …

Vascular adhesion molecule 1 and intercellular adhesion molecule 1 expression on rat liver cells after lipopolysaccharide administration in vivo. (1995 Dec)
vascular adhesion molecule 1 and intercellular adhesion molecule 1 expression on rat liver cells after lipopolysaccharide administration in vivo . during sepsis the infiltration of leukocytes plays a pivotal role in tissue damage . induction of septic shock results in an early accumulation of polymorphonuclear leukocytes in the liver ( after 3 hours ) , which is followed by an infiltration of mononuclear phagocytes ( after 30 hours ) . expression of adhesion molecules may contribute to the migration of leukocytes to the site of inflammation . therefore , in the present study we determined the expression of intercellular adhesion molecule 1 ( ICAM 1 ) and vascular adhesion molecule 1 ( VCAM 1 ) on hepatocytes , liver endothelial cells , and kupffer cells after lipopolysaccharide ( LPS ) treatment of rats in vivo . parenchymal cells showed no constitutive expression of VCAM 1 and the expression could not be upregulated by LPS treatment in vivo , whereas kupffer and endothelial cells had a low basal expression of VCAM 1 and this expression was increased 40 fold by LPS treatment in vivo . All three cell types showed a basal expression of ICAM 1 and the expression on endothelial liver cells of untreated rats was two times higher than the expression on parenchymal and kupffer cells . stimulation with LPS increased the expression of ICAM 1 2 . 5 times per parenchymal cells and approximately 4 times for endothelial and kupffer cells . It is concluded that the expression …

Reciprocal activation of leukocyte endothelial adhesion molecules in acute coronary syndromes. (2003 Sep)
reciprocal activation of leukocyte endothelial adhesion molecules in acute coronary syndromes . background : The acute coronary syndromes are associated with an intense inflammatory response and sustained leukocyte activation . This inflammatory state has been correlated with an adverse prognosis , but the source of this inflammation remains controversial , with evidence that it may arise either from the coronary vasculature or from the systemic endothelium . methods : levels of soluble cell adhesion molecules , and of their respective monocyte cell surface ligands , were measured in the peripheral serum of 21 patients presenting with acute coronary syndromes . soluble intercellular adhesion molecule 1 and soluble vascular cell adhesion molecule 1 were measured by enzyme linked immunosorbent assay and expression of the monocyte integrins cd11b ( Mac 1 ) and cd49d ( VLA 4 ) was measured by direct immunofluorescence using flow cytometry . results : High levels of the monocyte receptor cd11b ( 531 vs . 345 MFI , P 0 . 01 ) , and its soluble intercellular adhesion molecule 1 ( 329 vs . 232 ng / ml , P 0 . 01 ) , were noted in patients with acute coronary syndromes compared to healthy controls . conclusions : reciprocal activation of monocyte receptor ligands and endothelial adhesion molecules was found in the peripheral blood of patients with acute coronary syndromes . This may indicate a coordinated state of pro inflammatory upregulation with widespread activation of both leukocytes and endothelium and suggests a systemic rather …

Possible involvement of mononuclear cells stimulated with specific allergen from asthmatic patients in ICAM 1 expression on the eosinophilic cell … (1995 Dec)
possible involvement of mononuclear cells stimulated with specific allergen from asthmatic patients in ICAM 1 expression on the eosinophilic cell line . adhesion molecules , particularly intercellular adhesion molecule 1 ( ICAM 1 ) , recently have been considered to play a key role in inflammatory processes in asthma . Thus , from the point of view of cell interactions between mononuclear cells and eosinophils , we examined whether the supernatant of mononuclear cells ( MNC ) obtained from mite allergic asthmatic patients cultured with specific allergen is involved in ICAM 1 expression using an eosinophilic cell line ( EoL ) . ICAM 1 expression was induced by the supernatant of MNC from mite allergic asthmatic patients stimulated with mite allergen as well as by a combination of IL 3 , GM CSF , and IL 5 . Thus , we could conclude that some cytokines produced by specific allergen stimulated MNC in asthmatics might be involved in allergic inflammation through the induction of adhesion molecule expression such as ICAM 1 on eosinophils in asthma or allergic disorders .