Expression of intercellular adhesion molecule 1 and vascular adhesion molecule 1 in kidney of patients with lupus nephritis and membranoproliferative … (1995 Oct)
expression of intercellular adhesion molecule 1 and vascular adhesion molecule 1 in kidney of patients with lupus nephritis and membranoproliferative glomerulonephritis immunohistochemical and computer imaging analysis techniques were used to detect the expression of intercellular adhesion molecule 1 ( ICAM 1 ) and vascular adhesion molecule 1 ( VCAM 1 ) in the kidney of 25 patients with lupus nephritis ( LN ) type IV and in 15 patients with membranoproliferative glomerulonephritis ( MPGN ) type I . results showed that ICAM 1 and VCAM 1 expressions were increased significantly in the kidneys of patients with MPGN and LN and that ICAM 1 in the glomeruli of LN patients correlated well with glomerular endothelial cell proliferation . ICAM 1 and VCAM 1 may well take a role in the pathogenesis of LN and MPGN .

In situ expression of intercellular adhesion molecule 1 in human glomerulonephritis We conducted immunocytochemical analysis with in situ hybridization technique … (1995 Jul)
In situ expression of intercellular adhesion molecule 1 in human glomerulonephritis We conducted immunocytochemical analysis with in situ hybridization technique to investigate the presence and role of intercellular adhesion molecule 1 ( ICAM 1 ) in normal and diseased kidneys . A total of 64 renal biopsy specimens were classified in three groups according to the degree of cellular proliferation in glomerulus . group A presented no or mild proliferation ( n 38 ) . group B showed definite proliferation ( n 21 ) . group C had glomerular sclerosis ( n 5 ) . The ratio of cases exhibiting increased glomerular ICAM 1 expression in lupus nephritis ( 9 / 10 ) was significantly different from that in minimally changed disease ( 1 / 5 ) ( P 0 . 017 ) . glomerular ICAM 1 expression level correlated well with the cellular proliferation and infiltration in glomerulus ( B A : P 0 . 001 ; C B : P 0 . 05 ) . The percentage of cases with tubular ICAM 1 positivity in group B ( 81 . 0 ) and group C ( 100 ) was greater than that in group A ( 52 . 6 ) , which was also correlated with that of interstitial cell infiltration . In situ hybridization of 9 renal biopsies with digoxigenin labelled oligo probe proved immunocytochemical findings . We conclude that glomerular ICAM 1 expression level is correlated with inflammatory degree of glomerulus and that tubular ICAM 1 …

Image analysis for intercellular adhesion molecule 1 expression in MRI / lpr mice : effects of chinese herb medicine To … (1995 Jul)
image analysis for intercellular adhesion molecule 1 expression in MRI / lpr mice : effects of chinese herb medicine To clarify the role of intercellular adhesion molecule 1 ( ICAM 1 ) on pathogenesis of autoimmune lupus nephritis in MRL / lpr mice and the effects of chinese herb medicine ( stragalin ) , a computer image analysis system was used to study immunodepositions of ICAM 1 , immunoglobulins and C3 in renal tissue sections of the mice . ICAM 1 was found in the mesangial area and deposited along the glomerular capillary walls in MRL / lpr mice . The distribution intensity of ICAM 1 , immunoglobulins and C3 were significantly decreased after treatment with stragalin in form of decoction per os in the mice . We found that ICAM 1 might play an important role in pathogenesis of autoimmune lupus nephritis in MRL / lpr mice and chinese herb medicine has some inhibition effects on immunodepositions in renal tissue of MRL / lpr mice .

Primary culture of proximal tubular cells ( PTC ) from normal mouse kidney as an in vitro model to study … (1995 May)
primary culture of proximal tubular cells ( PTC ) from normal mouse kidney as an in vitro model to study mechanisms of development of tubulointerstitial nephritis . induction of ICAM 1 in PTC by antigen primed lymphocytes cultured PTC clearly expressed intercellular adhesion molecule 1 ( ICAM 1 ) under the influence of tubular basement membrane antigen ( TBM ) primed lymphocytes . these TBM primed lymphocytes also demonstrated a high cytotoxic activity against cultured PTC . A pure preparation of isolated PTC from BALB / c mouse kidney was brought into primary culture . PTC was prepared by the method of boogaard PJ et al , and our modification . briefly , kidney was perfused with buffer containing 0 . 08 ( w / v ) collagenase . The cortical tissue was then filtered through nylon gauze . viable PTC were separated from other materials by isopycnic centrifugation on a discontinuous nycodenz gradient . The confluent monolayer of PTC showed a typical epithelial morphology with cobblestone like cells in the center of the cell islands . typical dome formation was observed in PTC cultures . these cells also strongly expressed gamma glutamyl transpeptitase activity . coculture of PTC with syngeneic lymphocytes primed with TBM antigen induced ICAM 1 expression in PTC . The TBM primed lymphocytes had a cytotoxic activity without complement . however , neither virgin lymphocytes nor liver antigen primed lymphocytes had cytotoxic activity . This simple syngeneic experimental model may allow us further molecular biological examination …

Distinct patterns of expression of intercellular adhesion molecule 1 , vascular cell adhesion molecule 1 , and endothelial leukocyte adhesion … (1993 Oct)
distinct patterns of expression of intercellular adhesion molecule 1 , vascular cell adhesion molecule 1 , and endothelial leukocyte adhesion molecule 1 in renal disease . background : We have conducted an immunohistochemical analysis to investigate the presence of cellular adhesion molecules in normal and diseased human kidneys . experimental design : A total of 44 renal biopsies were classified in eight groups according to pathologic diagnosis . using immunohistochemistry , microscopical evaluation by two observers in a blinded fashion , and statistical analysis ( p 0 . 01 ) , significant changes in the expression of intercellular adhesion molecule 1 , vascular cell adhesion molecule 1 , and endothelial leukocyte adhesion molecule 1 were evaluated in diseased versus normal kidneys . results : small caliber vessels , such as peritubular and interstitial capillaries remained negative or showed decreased expression of vascular cell adhesion molecule 1 . upregulation of this molecule was seen only on the endothelium of interstitial arterioles and venules of diseased kidneys . expression of intercellular adhesion molecule 1 was significantly increased on parietal epithelium of glomeruli in membranous nephropathy and henoch schönlein purpura , on mesangium in henoch schönlein purpura , membranoproliferative glomerulonephritis and IgA nephropathy , and in small interstitial vessels in membranous nephropathy and membranoproliferative glomerulonephritis . interstitial infiltrates contained only few intercellular adhesion molecule 1 positive cells in henoch schönlein purpura and IgA nephropathy . kidneys with lupus nephritis and henoch schönlein purpura demonstrated an increase of endothelial leukocyte adhesion molecule 1 on …

Suppression of experimental crescentic type anti glomerular basement membrane ( GBM ) nephritis by fk506 ( tacrolimus hydrate ) in … (1996 Nov)
suppression of experimental crescentic type anti glomerular basement membrane ( GBM ) nephritis by fk506 ( tacrolimus hydrate ) in rats . The effect of fk506 ( tacrolims hydrate ) , an immunosuppressive agent produced by streptomyces tsukubaensis , on crescentic type anti glomerular basement membrane ( GBM ) nephritis in rats was investigated . When rats were treated with fk506 from 1 or 20 days after the anti GBM serum injection , fk506 inhibited the increase in urinary protein excretion . histological observation demonstrated that fk506 suppressed glomerular alterations . In the fk506 treated rats , antibody production and rat IgG and C3 deposits on the GBM were significantly less than those in the nephritic control group . fk506 treatment suppressed the accumulation of ED 1 positive cells , CD4 positive cells , CD8 positive cells , interleukin 2 ( IL 2 ) receptor positive cells , leukocyte function associated antigen 1 ( LFA 1 ) positive cells and intercellular adhesion molecule 1 ( ICAM 1 ) expression in nephritic glomeruli . however , in the in vitro study , fk506 failed to inhibit the up regulated ICAM 1 expression on endothelial cells in response to tumor necrosis factor ( TNF ) alpha . On the other hand , IL 2 production from the spleen cells isolated from nephritic rats treated with fk506 was lower than that in the nephritic control rats . these results suggest that fk506 is effective against crescentic type anti GBM nephritis and that the …

Intercellular adhesion molecule 1 ( ICAM 1 ) expression is upregulated in autoimmune murine lupus nephritis. (1990 Mar)
intercellular adhesion molecule 1 ( ICAM 1 ) expression is upregulated in autoimmune murine lupus nephritis . intercellular adhesion molecule 1 ( ICAM 1 ) is a cell surface protein regulating interactions among immune cells . To determine whether altered expression of ICAM 1 occurs in autoimmune lupus nephritis , we studied ICAM 1 expression in kidneys of normal and autoimmune MRL lpr and ( NZBX NZW ) F1 ( NZB / W ) mice . By immunoperoxidase staining , ICAM 1 is constitutively expressed at low levels in proximal tubules ( PT ) , endothelium and interstitial cells in normal C3H / FeJ mice . In nephritic MRL lpr and NZB / W kidneys , staining for ICAM 1 is increased in the PT , particularly in the brush border , and is prominent in the glomerular mesangium and the endothelium of large vessels . By western blot analysis , ICAM 1 is not detected in the urine of normal BALB / c and C3H / FeJ or autoimmune MRL lpr . By northern blot analysis , nephritic MRL lpr and NZB / W have a two to fivefold increase in steady state levels of ICAM 1 transcripts in the kidney as compared with normal or prenephritic mice . This is paralleled by an increase in MHC class II transcripts . In cultured PT cells , ICAM 1 is expressed at basal levels in PT and is increased by the cytokines interferon gamma , IL 1 alpha , and …

Dietary omega 3 lipids delay the onset and progression of autoimmune lupus nephritis by inhibiting transforming growth factor beta mRNA … (1995 Dec)
dietary omega 3 lipids delay the onset and progression of autoimmune lupus nephritis by inhibiting transforming growth factor beta mRNA and protein expression . The present study was carried out to test whether transforming growth factor beta ( TGF beta ) plays a pathological role in the induction or progression of glomerulonephritis in a murine model of systemic lupus erythematosus ( SLE ) , and whether dietary supplementation with fish oil ( FO ) can modulate the expression of TGF beta . weanling female ( NZB x NZW ) F1 ( B / W ) mice were divided into three groups . One group was fed an unmanipulated diet ( lab . chow ; LC ) and the other two groups were fed a nutritionally adequate semipurified diet supplemented with 10 CO or FO . Both water and food were provided ad libitum . proteinuria and serum anti dsdna antibody levels were measured to assess disease progression . Mice were killed at 3 . 5 and 6 . 5 months of age and renal mRNA levels for TGF beta isoforms , fibronectin 1 ( FN 1 ) and intercellular adhesion molecule 1 ( ICAM 1 ) were studied by northern blot analysis . TGF beta 1 protein levels were also examined in kidneys by western blot analysis . Our results indicate that at 3 . 5 months of age , when urinary protein levels were undetectable and very low levels of anti dsdna were detected , no mRNA signal could …

Macrophage colony stimulating factor ( M CSF ) enhances proteinuria and recruitment of macrophages into the glomerulus in experimental murine … (1996 Dec)
macrophage colony stimulating factor ( M CSF ) enhances proteinuria and recruitment of macrophages into the glomerulus in experimental murine nephritis . In this study , we examined the effects of macrophage colony stimulating factor ( M CSF ) on glomerular macrophages in lipopolysaccharide ( LPS ) induced murine nephritis . Mice injected intraperitoneally with either M CSF plus LPS , LPS alone , M CSF alone or saline every day for 8 days were examined for the degree of urine albumin excretion and lymphocyte function associated antigen 1 positive ( LFA 1 ) cells in peripheral blood as well as renal pathology . From our results , LPS or M CSF combined with LPS emphasized the degree of proteinuria , glomerular deposition of immunoglobulins and mesangial proliferation , associated with accumulation of macrophages in the glomeruli . however , in immunohistological examination of kidneys from these nephritic mice , neither intercellular adhesion molecule 1 ( ICAM 1 ) , which may play an important role in the recruitment of macrophages into glomeruli , M CSF receptor nor the number of LFA 1 cells in peripheral blood was enhanced by M CSF . On the other hand , M CSF alone induced neither proteinuria nor any pathological changes and did not increase the number of glomerular Mac 1 cells above that in saline treated controls . these results indicate that M CSF does not directly cause glomerulonephritis but might participate in accelerating the glomerular inflammatory process by stimulating a potent …

Expression of adhesion molecules in kidney with experimental chronic obstructive uropathy : the pathogenic role of ICAM 1 and VCAM … (2000 Sep)
expression of adhesion molecules in kidney with experimental chronic obstructive uropathy : the pathogenic role of ICAM 1 and VCAM 1 . background : chronic obstructive uropathy induced by maintained unilateral ureter ligation in the rat is characterized morphologically by interstitial inflammation , interstitial fibrosis , and tubular atrophy . infiltrating mononuclear inflammatory cells , particularly T lymphocytes and macrophages , may contribute to the progression of this lesion by mediating tubular injury and by the activation of interstitial fibroblasts , with resultant tubular atrophy and interstitial fibrosis , respectively . altered expression and activation of adhesion molecules by leukocytes , vascular endothelial cells , and parenchymal cells likely contributes both to the infiltration of inflammatory cells into the tubulointerstitial compartment and to the interaction of activated inflammatory cells with parenchymal cells . methods : In the current study , we examined changes in the expression of intercellular adhesion molecule 1 ( ICAM 1 ) and vascular cell adhesion molecule 1 ( VCAM 1 ) in a 90 day model of maintained unilateral ureter ligation in male sprague dawley rats . results : Rat kidneys showed constitutive expression of ICAM 1 mRNA and constitutive immunostaining for ICAM 1 in peritubular capillaries , glomeruli , and a small percentage of cortical tubules . ureter ligation resulted in a rapid increase in ICAM 1 mRNA , which was almost 2 fold greater than those of the contralateral and control kidneys as early as 3 h and which was maintained at a 4 …

Antinephritis effect of anti ICAM 1 monoclonal antibody and anti LFA 1 monoclonal antibody on accelerated type anti GBM nephritis … (1994 Apr)
antinephritis effect of anti ICAM 1 monoclonal antibody and anti LFA 1 monoclonal antibody on accelerated type anti GBM nephritis in rats The involvement of adhesion molecules in the accumulation of inflammatory cells in the glomeruli and subsequent glomerular injury was examined in accelerated type anti GBM nephritis in rats . This animal model is known to be manifested by progressive and irreversible glomerular damage with rapid influx of monocytes / macrophages into the glomeruli . Up regulated expression of intercellular adhesion molecule 1 ( ICAM 1 ) was observed exclusively in the glomerular endothelial cells in this model . therefore we examined the effect of ICAM 1 and lymphocyte function associated antigen 1 ( LFA 1 ) in the pathogenesis of this nephritis . Four groups of rats with accelerated type anti GBM nephritis were investigated . The first group was the control group injected with normal mouse IgG 2mg / Kg . The second group was administered mouse anti rat ICAM 1 monoclonal antibody ( 1A 29 ) 2 mg / Kg . The third group was administered mouse anti rat LFA 1 beta monoclonal antibody ( WT 3 ) 2mg / Kg . The fourth group received both 1A 29 1mg and WT 3 1mg / Kg . proteinuria and the influx of monocytes / macrophage in this model were decreased after the administration of 1A 29 or WT 3 . simultaneous administration of both antibodies showed intense suppression of the proteinuria and marked reduction of the …

Expression of cell adhesion molecules in tubulointerstitial nephritis associated with sjögren s syndrome. (1998 May)
expression of cell adhesion molecules in tubulointerstitial nephritis associated with sjögren s syndrome . The tissue distribution of cellular adhesion molecules ( ICAM 1 , ELAM 1 , VCAM 1 ) was studied in specimens from six normal human kidneys and in six biopsies from kidneys with tubulointerstitial nephritis associated with sjögren s syndrome . In addition , the expression of cellular adhesion molecules was examined both in four renal biopsies from cases of tubulointerstitial nephritis of diverse pathogenesis and in six lip biopsies from cases of sjögren s syndrome . ICAM 1 was expressed on vascular endothelial cells in normal kidneys , in all specimens of tubulointerstitial nephritis and in salivary glands . On tubular epithelial cells , ICAM 1 appeared slightly in normal kidneys ; otherwise tubular epithelial ICAM 1 was observed in and around the foci of cellular infiltration in all cases of tubulointerstitial nephritis . ELAM 1 and VCAM 1 were observed on the newly generated vessels in massive cellular infiltrates in some cases of tubulointerstitial nephritis associated with sjögren s syndrome ; by contrast , they were not seen in normal kidneys and in cases of tubulointerstitial nephritis of diverse pathogenesis . In the lip biopsies from salivary glands , ICAM 1 was observed on ductal epithelial cells in and around the foci of cellular infiltration , and ELAM 1 and VCAM 1 occasionally appeared on the newly generated vessels in massive cellular infiltrates . chronic and progressive inflammation may be facilitated by such ELAM …

The critical role of intercellular adhesion molecule 1 in masugi nephritis in rats. (1996 Nov)
The critical role of intercellular adhesion molecule 1 in masugi nephritis in rats . intercellular adhesion molecule 1 ( ICAM 1 , CD54 ) , an adhesion molecule of the immunoglobulin superfamily , is an endothelial cell surface ligand for such leukocyte integrins as lymphocyte function associated molecule 1 ( LFA 1 , cd11a / CD18 ) , Mac 1 ( cd11b / CD18 ) and CD43 . these molecules mediate adhesive interactions between leukocytes and endothelial cells and are critically involved in infiltration of leukocytes into inflammatory lesions . We examined the expression of ICAM 1 in renal tissues of masugi nephritis rats and directly examined the role of ICAM 1 by administration of neutralizing monoclonal antibodies ( MAbs ) to rat ICAM 1 , LFA 1 alpha subunit ( LFA 1 alpha ) , beta subunit ( LFA 1 beta ) and Mac 1 alpha subunit ( Mac 1 alpha ) . within 3 h after injection of nephrotoxic serum , increased expression of ICAM 1 was detected in the glomeruli by in situ hybridization and an immunofluorescence study . proteinuria was significantly suppressed by the MAbs against ICAM 1 , Mac 1 alpha and LFA 1 beta . neutrophil infiltration into the glomeruli was significantly prevented by injection of the MAbs against ICAM 1 , LFA 1 alpha and LFA 1 beta . these results indicate that both ICAM 1 / LFA 1 and ICAM 1 / Mac 1 pathways are involved in neutrophil infiltration into the …

Role of leucocyte adhesion molecules in aminonucleoside of puromycin ( PAN ) associated interstitial nephritis. (1997 May)
Role of leucocyte adhesion molecules in aminonucleoside of puromycin ( PAN ) associated interstitial nephritis . Rats receiving a single dose ( 10 mg / l00 g body wt ) of PAN develop severe proteinuria and acute interstitial nephritis . To investigate the mechanisms involved in interstitial leucocyte accumulation , we examined the expression of adhesion molecules on kidney tissue sections as well as on endothelial cell cultures . We also performed in vivo treatments with antibodies against adhesion molecules . enhanced expression of intercellular adhesion molecule 1 ( ICAM 1 ) was found on day 7 of the disease , when interstitial nephritis was first detected . Also , rat endothelial cells in culture showed maximal expression of ICAM 1 in the presence of 10 ( 9 ) 10 ( 11 ) M PAN . adhesion of peripheral blood mononuclear cells ( PBMC ) on kidney sections from PAN treated rats was highest on day 7 ( 3 . 05 / 0 . 7 ( mean / s . e . m . ) ; controls 0 . 75 / 0 . 5 ) . Such increased adhesion was notably blocked after PAN treated rat kidney sections were incubated with anti ICAM 1 MoAb ( 0 . 9 / 0 . 4 ) . In addition , adhesiveness of PBMC to PAN stimulated endothelial cells in culture was enhanced ( 25 / 2 . 5 ; non stimulated cells 13 / 3 . 1 ) . The addition of …

Expression of the intercellular adhesion molecule 3 ( ICAM 3 ) in human renal tissue with relation to kidney transplants … (1995 Dec)
expression of the intercellular adhesion molecule 3 ( ICAM 3 ) in human renal tissue with relation to kidney transplants and various inflammatory diseases . adhesion molecules are important for immune regulatory mechanisms concerning antigen presentation , lymphocyte activation , localisation and migration as well as effector target cell interactions in inflammatory processes . The immunohistochemical expression of ICAM 3 , a recently cloned new member of the immunoglobulin family which also binds leucocyte function antigen 1 ( LFA 1 ) , was examined in 80 needle core biopsies from 35 renal allografts , 7 patients with mesangioproliferative glomerulonephritis , 5 patients with extracapillary glomerulonephritis , 4 patients with interstitial nephritis and 5 patients with diabetic nephropathy and 20 normal kidneys . In all types of lesions ICAM 3 was constitutively expressed on the majority of infiltrating leucocytes without detectable upregulation or presentation on possible target structures during inflammation indicating its possible role to be mainly in initiation of the inflammatory response .

Correlation of renal tubular epithelial cell derived interleukin 18 up regulation with disease activity in MRL faslpr mice with autoimmune … (2002 Nov)
correlation of renal tubular epithelial cell derived interleukin 18 up regulation with disease activity in MRL faslpr mice with autoimmune lupus nephritis . objective : MRL Fas ( lpr ) mice spontaneously develop an autoimmune disease that mimics systemic lupus erythematosus in humans . infiltrating T cells expressing interferon gamma ( ifngamma ) are responsible for the autoimmune kidney destruction in MRL Fas ( lpr ) mice , and interleukin 18 ( IL 18 ) released by mononuclear phagocytes stimulates T cells to produce the ifngamma . since MRL Fas ( lpr ) T cells are characterized by an overexpression of the IL 18 receptor accessory chain , we sought to determine the impact of IL 18 on the progression of lupus nephritis in MRL Fas ( lpr ) mice . methods : IL 18 expression in sera and kidney tissues from MRL Fas ( lpr ) mice was determined by enzyme linked immunosorbent assay ( elisa ) , reverse transcription polymerase chain reaction ( RT PCR ) , immunohistochemistry , and western blotting . IL 18 production by primary cultured tubular epithelial cells ( TECs ) from MRL Fas ( lpr ) and BALB / c mice were examined by RT PCR , elisa , and western blotting . The interactions of TEC derived IL 18 and MRL Fas ( lpr ) T cells were studied in coculture assays . IL 18 related effects on TEC viability and adhesion molecule expression were determined by fluorescence activated cell sorting and …

Monocyte response to Th1 stimulation and effector function toward human mesangial cells are not impaired in patients with lupus nephritis. (2003 Feb)
monocyte response to Th1 stimulation and effector function toward human mesangial cells are not impaired in patients with lupus nephritis . monocytes / macrophages activated by Th1 stimulation such as interferon gamma ( IFN gamma ) and CD40 ligand ( cd40l ) infiltrate the kidney and play a critical role in the progression of lupus nephritis ( LN ) . We examined the monocyte response to Th1 stimulation and their effector function toward activating renal resident cells in patients with LN . following stimulation with IFN gamma granulocyte macrophage colony stimulating factor ( GM CSF ) / recombinant cd40l the production of tumor necrosis factor alpha and IL 12 p70 by PBMC was significantly higher in LN patients . In coculture experiments employing activated monocytes and human mesangial cells , there was a trend toward higher monocyte chemoattractant protein 1 production by lupus monocytes compared to normal controls . basal expression of CD40 , ICAM 1 , and STAT 1 was significantly higher in monocytes from LN patients , suggesting ongoing activation . monocyte response to IFN gamma , as accessed by intercellular adhesion molecule 1 upregulation and phosphorylation of STAT 1 , was comparable between the two groups . Thus , in contrast to earlier reports , Th1 dependent monocyte activation is not impaired . In this disease activated monocytes appear to be fully capable of inducing renal injury .

Butein ( 3 , 4 , 2 , 4 tetrahydroxychalcone ) ameliorates experimental anti glomerular basement membrane antibody associated glomerulonephritis … (1997 Jun)
butein ( 3 , 4 , 2 , 4 tetrahydroxychalcone ) ameliorates experimental anti glomerular basement membrane antibody associated glomerulonephritis ( 3 ) . The antinephritic effects of butein ( 3 , 4 , 2 , 4 tetrahydroxychalcone ) on original type anti glomerular basement membrane antibody associated glomerulonephritis in rats were investigated . butein was given to anti glomerular basement membrane antibody associated glomerulonephritic rats for 15 days after the induction of nephritis . butein prevented proteinuria and histological alterations . The up regulation of intercellular adhesion molecule 1 ( ICAM 1 ) expression and increase in leukocyte function associated antigen 1 ( LFA 1 ) positive cells in nephritic glomeruli significantly declined with butein treatment . In the further investigation to clarify the effects of butein on ICAM 1 expression , human umbilical vein endothelial cells were treated with butein in the presence of tumor necrosis factor alpha ( TNF alpha ) or phorbol 12 myristate 13 acetate ( PMA ) . butein prevented the up regulation of ICAM 1 expression mediated by TNF alpha or PMA on human umbilical vein endothelial cells in a dose dependent manner . When human umbilical vein endothelial cells or neutrophils were treated with butein , the adhesion of neutrophils to human umbilical vein endothelial cells was suppressed . these data suggest that the antinephritic action of butein is due to inhibition of intraglomerular accumulation of leukocytes through the prevention of the up regulation of ICAM 1 and the inhibition of a …

Glomerular ICAM 1 expression related to circulating TNF alpha in human glomerulonephritis. (1997 Oct)
glomerular ICAM 1 expression related to circulating TNF alpha in human glomerulonephritis . To clarify the in vivo involvement of cellular adhesion molecules and cytokines in human glomerulonephritis , we have investigated the glomerular and interstitial expression of intercellular adhesion molecule 1 ( ICAM 1 ) in 69 kidney biopsy specimens by immunohistochemical methods and its correlation with serum bioactive tumor necrosis factor alpha ( TNF alpha ) and soluble ICAM 1 ( sicam 1 ) levels in 43 cases . In normal controls , glomerular ICAM 1 expression and serum TNF alpha and sicam 1 levels showed a mean score of 1 . 0 ( n 7 ) and were 12 . 1 / 1 . 5 and 187 / 5 ng / ml ( mean / SEM , n 25 ) , respectively . ICAM 1 was positive in 68 kidneys except in 1 patient with membranous nephropathy at various degrees in glomeruli and in 72 of peritubular capillaries or venules in the interstitium . serum bioactive TNF alpha levels increased in the patients with IgA nephropathy , purpura nephritis , and lupus nephritis ( LN ) ( 18 . 9 / 4 . 1 , 32 . 6 / 13 . 3 , and 20 . 9 / 3 . 5 pg / ml ) and were positively correlated with the grade of glomerular ICAM 1 expression ( n 43 , r 0 . 57 , p 0 . 001 ) , endocapillary proliferation with exudative lesions …

Inflamed site specific delivery of bone marrow derived cells carrying IL 1Ra. (2002 Oct)
inflamed site specific delivery of bone marrow derived cells carrying IL 1Ra . A gene delivery system using bone marrow derived cd11b ( ) CD18 ( ) cells and their interaction with adhesion molecules was established . after transplantation into mice , these vehicle cells may be recruited into glomeruli upon lipopolysaccharide ( LPS ) treatment , and the number of recruited cells corresponds to the expression of intercellular adhesion molecule 1 ( ICAM 1 ) in glomeruli . using this system , interleukin 1 receptor antagonist ( IL 1Ra ) was delivered into animal models of glomerulonephritis evoked by anti glomerular basement membrane antibody ( anti GBM nephritis ) . urinary albumin excretion and the serum creatinine level were significantly elevated after anti GBM antibody injection in mock treated mice , whereas they were suppressed in the IL 1Ra treated mice . histological analysis revealed that glomerular injuries were also suppressed in IL 1Ra treated mice . We further confirmed that specificity for inflamed glomeruli was significantly enhanced by a combination of the Cre / loxP system with the IL 1beta promoter . these data suggested that our novel system may be used as a therapeutic intervention for glomerulonephritis .