Endothelial adhesion molecules and their role in inflammation. (1993 Jul)
endothelial adhesion molecules and their role in inflammation . The emigration of leukocytes such as neutrophils into inflammatory sites requires adhesion to the endothelium of small venules . The initial adhesive event is margination characterized by rolling of neutrophils along the luminal surface of the endothelium . Each member of the selectin family of adhesion molecules has been shown to support neutrophil rolling under conditions of flow . E selectin is synthesized by endothelial cells following cytokine stimulation , P selectin is rapidly mobilized from weibel palade bodies to the endothelial cell surface following stimulation with agents such as histamine , and L selectin is constitutively expressed on the surface of leukocytes . Each selectin functions primarily as a lectin , recognizing carbohydrate structures on the leukocyte or endothelial cell surface . Once the marginated neutrophil forms a stationary adhesion with endothelial cells , it is stimulated by chemotactic factors to downregulate the selectin based adhesion and upregulate adherence dependent on beta 2 integrins , principally cd11a / CD18 ( LFA 1 ) and cd11b / CD18 ( Mac 1 ) . these adhesion molecules interact with intercellular adhesion molecule 1 ( ICAM 1 ) and possibly other structures on the endothelial cell , and the leukocyte rapidly emigrates into surrounding tissue . transendothelial migration in vitro is markedly inhibited by monoclonal antibodies against CD18 integrins or ICAM 1 . monoclonal antibodies against the selectins , CD18 , cd11a , cd11b , and ICAM 1 have all been shown to …

Inflammatory cells and oxygen radicals. (2005 Aug)
inflammatory cells and oxygen radicals . At sites of inflammation , multiple inflammatory cells including eosinophils , neutrophils , and macrophages are capable of generating reactive oxygen species ( ROS ) , which can contribute to development of various diseases . In case of allergic inflammation , for example , the lung cells obtained by bronchoalveolar lavage ( BAL ) following antigen challenge generates superoxide anion at nanomolar concentrations . eosinophils obtained from BAL following a segmental allergen challenge generate more superoxide anion than eosinophils obtained from the peripheral circulation . Such ROS may contribute not only to tissue injury but also to inflammatory reactions . For example , hydrogen peroxide can stimulate both neutrophil and eosinophil adhesion as an autocrine or paracrine mediator via the upregulation of beta2 integrin . furthermore , ROS may alter morphological or functional properties of endothelial cells , including permeability and adhesion molecule expression . Thus , ROS can promote adhesive interaction between inflammatory and endothelial cells , which could culminate in manifestations of inflammatory diseases such as bronchial asthma .

Endothelial cells in inflammation and angiogenesis. (2005 Aug)
endothelial cells in inflammation and angiogenesis . endothelial cells are involved in leukocyte extravasation underlying inflammation . A number of adhesion molecules play a role in leukocyte endothelial interactions . New vessel formation , termed angiogenesis , is also crucial for leukocyte extravasation . The outcome of neovascularization is highly dependent on the balance or imbalance between angiogenic mediators and inhibitors . there have been several attempts to therapeutically interfere with the cellular and molecular mechanisms , such as leukocyte endothelial cell adhesion and angiogenesis . Most studies have been performed using animal models of various types of inflammation , such as arthritis . In addition , a very limited number of human clinical trials gave promising results . In this review , authors summarize the most relevant information on adhesion molecules , as well as angiogenic and angiostatic agents . In addition , further perspectives of anti adhesive and anti angiogenic therapy are also discussed . specific targeting of pathological endothelial function including adhesion and angiogenesis , may be useful for the future management of various inflammatory diseases .

Platelet / endothelial cell adhesion molecule 1 ( pecam 1 ) expression by human brain microvessel endothelial cells in primary … (1997 Mar)
platelet / endothelial cell adhesion molecule 1 ( pecam 1 ) expression by human brain microvessel endothelial cells in primary culture . pecam 1 expression was investigated in primary cultures of human brain microvessel endothelial cells ( hbmec ) . hbmec constitutively express pecam 1 along their apical cell surface , advancing processes and on the basal surface at points of contact with the extracellular matrix . surface expression is not altered by cytokine or lipopolysaccharide treatment . This distribution may mediate cell cell contract and migration during angiogenesis and hbmec leukocyte interactions in CNS inflammation .

Role of intra cellular adhesion molecule 1 ( ICAM 1 ) and its soluble form ( sicam ) in chronic … (2004 Jun)
Role of intra cellular adhesion molecule 1 ( ICAM 1 ) and its soluble form ( sicam ) in chronic airway inflammation mucosal inflammation is the feature of both bronchial asthma and allergic rhinitis with evident of tissue eosinophilia , mast cells , eosinophils and T lymphocytes activation . The initial phase of cell recruitment is the margination and adhesion of leucocytes to the endothelium , prior to their transendothelial migration under a directed chemotactic stimulus . This adhesion occurs through specific ligand receptor couplets involving leucocyte endothelial adhesion molecules . One of these cell adhesion molecules is ICAM 1 , an important early marker of immune activation and response . Its ligand , leukocyte function associated antigen one ( LFA 1 ) is expressed on neutrophils , eosinophils and T cells . ICAM 1 was found to be expressed on epithelial and endothelial cells in rhinitis patients and in bronchial biopsies obtained from asthmatics also after allergen challenge . circulating forms of these adhesion molecules have been identified in the peripheral blood , bronchoalveolar lavage ( BAL ) fluid and nasal lavage in patients with asthma and rhinitis . systemic and local up regulation of sicam 1 suggests a function role for this soluble form of ICAM in the allergic inflammation .

Human / severe combined immunodeficient mouse chimeras. (1993 Apr)
human / severe combined immunodeficient mouse chimeras . An experimental in vivo model system to study the regulation of human endothelial cell leukocyte adhesion molecules . The ability of circulating white blood cells to enter inflamed tissues is mediated by specific cell adhesion molecules thought to be expressed in a programmed and sequential manner to form an adhesion cascade . because of the complexity of this process , it is becoming increasingly important to develop in vivo models . Two major problems have limited the utility of current animal models . The first is the inability of many of the antibodies developed against cell adhesion molecules in human cell culture models to cross react in animals . The second is the uncertainty in extrapolating animal ( particularly rodent ) findings to humans . To circumvent these problems , full thickness human skin grafts were transplanted onto immunodeficient ( severe combined immunodeficient ) mice . after 4 6 wk , the transplanted skin grafts closely resembled normal skin histologically and maintained their human vasculature as determined by immunohistochemical staining with human specific endothelial cell markers . intradermal injection of tumor necrosis factor alpha resulted in the reversible upregulation of the leukocyte endothelial adhesion molecules E selectin , vascular cell adhesion molecule 1 , and intercellular adhesion molecule 1 , and in an active inflammatory reaction with migration of murine leukocytes into cytokine injected areas . these results indicate that the severe combined immunodeficient mouse / human skin transplant model provides a …

Dynamic expression of L selectin in cell to cell interactions between neutrophils and endothelial cells in vitro. (1998 Sep)
dynamic expression of L selectin in cell to cell interactions between neutrophils and endothelial cells in vitro . neutrophil endothelial cell interactions are regulated by cell adhesion molecules and their cognate ligands . It has been proposed that L selectin and Mac 1 ( cd11b / CD18 ) , two neutrophil adhesion receptors , have sequential roles in neutrophil extravasation during inflammation . In this model , L selectin mediates rolling and initial adherence of neutrophils to endothelial cells , while Mac 1 strengthens this initial adherence and also facilitates migration of neutrophils through endothelial cells . L selectin and Mac 1 expression are known to be inversely regulated . Here an in vitro culture system has been developed to investigate in situ expression of L selectin during cell to cell interactions between neutrophils and endothelial cell monolayers by confocal immunofluorescence analysis . neutrophils underwent profound cell shape change from round to polarized cell morphology with pseudopod formation after 5 to 15 min coculture with IL 1 stimulated human endothelial cells . L selectin was redistributed to the pseudopod of the polarized neutrophils in correlation with such cellular changes . during initial cell attachment , neutrophils bound to IL 1 stimulated endothelial cells expressed a high level of L selectin in a polarized pattern . L selectin expression decreased over time during neutrophil endothelial cell interactions .

Expression of cell adhesion molecules in the lungs of patients with idiopathic pulmonary fibrosis. (1995 Aug)
expression of cell adhesion molecules in the lungs of patients with idiopathic pulmonary fibrosis . idiopathic pulmonary fibrosis ( IPF ) is a chronic inflammatory disorder restricted to the lungs . leukocyte entry into the area of inflammation is regulated , at least partly , by endothelial expression of leukocyte selective cell adhesion molecules ( CAMs ) . To investigate the relevance of these CAMs to the accumulation of leukocytes in IPF , we examined the expression of E selectin ( endothelial leukocyte adhesion molecule 1 ; ELAM 1 ) , intercellular adhesion molecule 1 ( ICAM 1 ) , and vascular cell adhesion molecule 1 ( VCAM 1 ) by immunohistochemistry in lung tissue from nine patients with IPF and five nonsmoking normal subjects . The results demonstrated that in normal lungs , ICAM 1 was weakly expressed on endothelial cells , but neither E selectin nor VCAM 1 was detected . In the lungs of patients with IPF , E selectin expression on endothelial cells was restricted to honeycombing regions . endothelial expression of ICAM 1 was increased throughout the tissue , but VCAM 1 was not detected in IPF . The distribution of leukocytes in lungs with IPF consisted of mostly lymphocyte accumulation in the interstitium and neutrophil accumulation within the airspaces of honeycomb regions . these results suggest that E selectin may play a role in the recruitment of neutrophils in regions of honeycombing and that ICAM 1 may play a role in lymphocyte recruitment into …

An inhibitor of ikappab kinase , BMS 345541 , blocks endothelial cell adhesion molecule expression and reduces the severity of … (2004 Mar)
An inhibitor of ikappab kinase , BMS 345541 , blocks endothelial cell adhesion molecule expression and reduces the severity of dextran sulfate sodium induced colitis in mice . objective : inflammatory bowel diseases such as ulcerative colitis and crohn s disease are characterized by chronic relapsing inflammation . The transcription of many of the proteins which mediate the pathogenesis in inflammatory bowel disease ( e . g . , tnfalpha , ICAM 1 , VCAM 1 ) is NF kappab dependent . ikappab kinase is critical in transducing the signal inducible activation of NF kappab and , therefore , represents a potentially promising target for the development of novel agents to treat inflammatory bowel disease and other inflammatory diseases . results : Here we show that BMS 345541 , a highly selective inhibitor of ikappab kinase , inhibited the tnfalpha induced expression of both ICAM 1 and VCAM 1 in human umbilical vein endothelial cells at the same concentration range as cytokine expression is inhibited in monocytic cells ( IC ( 50 ) congruent with 5 microm ) . against dextran sulfate sodium induced colitis in mice , BMS 345541 administered orally at doses of 30 and 100 mg / kg was effective in blocking both clinical and histological endpoints of inflammation and injury . conclusion : This represents the first example of an inhibitor of ikappab kinase with anti inflammatory activity in vivo and indicates that inhibitors of IkB kinase show the promise of being highly efficacious in inflammatory …

Expression of the adhesion molecules ICAM 1 , VCAM 1 , LFA 1 and VLA 4 in the skin is … (1999 Apr)
expression of the adhesion molecules ICAM 1 , VCAM 1 , LFA 1 and VLA 4 in the skin is modulated in progressing stages of chronic venous insufficiency . In inflammation and wound healing , dynamic changes in cell adhesion and migration are fundamental properties of the cells involved . disturbed interaction of leukocytes with microvascular endothelial cells has been proposed to be a central pathogenic factor in chronic venous insufficiency . This disease may therefore serve to elucidate dysregulated modulation of adhesion molecule expression in conditions of chronic inflammation and impaired wound healing . In this study , we determined how the expression of ICAM 1 / VCAM 1 on endothelial cells and their ligands LFA 1 / VLA 4 on leukocytes is modulated in skin of progressing stages of chronic venous insufficiency . immunohistochemical staining of skin biopsies revealed an increase in the expression of ICAM 1 and VCAM 1 on endothelial cells in an early stage of venous disease such as stasis dermatitis . Such protein expression correlated with an increase of corresponding mRNA in skin biopsies . expression of these CAMs on endothelial cells was accompanied by the occurrence of a marked perivascular infiltration of leukocytes , which expressed increased levels of LFA 1 and VLA 4 . In progressing stages of chronic venous insufficiency , characterized by hyperpigmentation and lipodermatosclerosis , which precede skin ulceration , all these CAMs remained upregulated on endothelial cells and infiltrating leukocytes . Our findings indicate that following an initial …

Efficiency of lymphocytes adhesion to endothelial cells of distinct tissue origin from children with asthma lymphocytes extravasation is determined by … (2004 Jun)
efficiency of lymphocytes adhesion to endothelial cells of distinct tissue origin from children with asthma lymphocytes extravasation is determined by their adhesion to the endothelial cells . The process goes along with high degree selectivity and is limited to lymph nodes and lymphatic tissue . It comes to uncontrolled extravasation to the tissues of involved organs in diseases with allergic origin as well in inflammation . Most information concerning adhesive interactions comes from researches dealing with normal lymphocytes . however , this phenomenon plays also crucial role in inflammation , metastasis and other pathologies . The aim of the work was to search for the possible distinct efficacy of adhesive interactions between peripheral blood lymphocytes from children with asthma and endothelial cells isolated from lung , skin and intestine . isolated peripheral blood leukocytes were overlayered into endothelial cell monolayer . after washing the unbound cells , adhering lymphocytes were collected with endothelial cells . For quantifying the percentage of the particular cell populations cytofluorometric method was applied . The results are presented as a number of adhering lymphocytes per one endothelial cell . It has been shown that lymphocytes from asthmatic children have significantly greater adhesive potential towards endothelial cell lines from lungs and skin as compared with adhesion to endothelium of intestine origin . however , their B lymphocytes subpopulation demonstrated significantly higher percentage of cells adhering to all endothelial cell lines tested , as compared with B lymphocytes from normal controls . there were not statistically different …

4 O methylgallic acid down regulates endothelial adhesion molecule expression by inhibiting NF kappab DNA binding activity. (2006 Nov)
4 O methylgallic acid down regulates endothelial adhesion molecule expression by inhibiting NF kappab DNA binding activity . We here investigated the functional effect of 4 O methylgallic acid ( 4 OMGA ) , a major metabolite of gallic acid abundant in red wine , on vascular inflammation and its action mechanism . 4 OMGA inhibited the expression of intercellular adhesion molecule 1 ( ICAM 1 ) and vascular cell adhesion molecule 1 ( VCAM 1 ) in human umbilical vein endothelial cells ( huvecs ) stimulated with tumor necrosis factor alpha ( TNF alpha ) , resulting in the suppression of leukocyte adhesion to huvecs . In addition , 4 OMGA inhibited the promoter activities of ICAM 1 and VCAM 1 and the activity of nuclear factor kappab ( NF kappab ) without affecting cytosolic ikappab kinase ( IKK ) activation , inhibitor of kappab ( ikappab ) phosphorylation and degradation , and nuclear translocation of NF kappab . This compound did not alter nitric oxide ( NO ) generation , but inhibited reactive oxygen species ( ROS ) production in TNF alpha stimulated huvecs , suggesting that NO and ROS are not involved in 4 OMGA mediated inhibition of NF kappab activity . moreover , 4 OMGA directly blocked the binding activity of NF kappab to its consensus DNA oligonucleotide , when pre incubated with the nuclear extract from TNF alpha stimulated huvecs , but not with the oligonucleotide alone . This inhibition was completely abolished by the …

Direct contact between human peripheral blood mononuclear cells and renal fibroblasts facilitates the expression of monocyte chemoattractant protein 1. (2003 Jul)
direct contact between human peripheral blood mononuclear cells and renal fibroblasts facilitates the expression of monocyte chemoattractant protein 1 . background : Cell to cell interaction is thought to be an important feature of a variety of biological processes . As far as the proinflammatory process is concerned , the interaction between mesangial cells and monocytes / macrophages induces the expression of monocyte chemoattractant protein 1 ( MCP 1 ) , and this may play a role in glomerulonephritis . In this study , we investigated whether the cell to cell interaction between immune cells and renal fibroblasts induces MCP 1 gene expression , which may be involved in interstitial inflammation in the kidney . methods : human renal fibroblast cell lines , tNKF ( from a normal kidney ) and tfkif ( from a kidney with fibrosis ) , and peripheral blood mononuclear cells ( PBMC ) were used to assess the effect of cell to cell contact on the expression of MCP 1 mRNA in the fibroblasts . The expression of the MCP 1 gene in the fibroblasts was also examined after stimulation with tumor necrosis factor alpha ( TNF alpha ) and the culture supernatant from PBMC . RT PCR was used to detect MCP 1 mRNA expression . neutralizing antibodies to intercellular adhesion molecule 1 ( ICAM 1 ) and vascular endothelial adhesion molecule 1 ( VCAM 1 ) were used to block the cell to cell contact between the fibroblasts and PBMC . results : …

Role of P selectin in radiation induced intestinal inflammatory damage. (2001 Apr)
Role of P selectin in radiation induced intestinal inflammatory damage . The aims of our study were to characterize the dose and time dependent changes in endothelial P selectin expression and the role of this adhesion molecule as a mediator of radiation induced inflammation . For that purpose , endothelial P selectin expression was measured by the radiolabeled antibody technique in control and irradiated mice at 2 , 6 , and 24 hr following abdominal irradiation with 4 or 10 Gy ; leukocyte endothelial cell interactions were assessed using intravital microscopy in intestinal venules following irradiation at the aforementioned doses and times in c57bl / 6 and P selectin deficient mice . In wild type mice , radiation induced a time and dose dependent up regulation of P selectin and a significant increase in the flux of rolling leukocytes 2 hr after irradiation . irradiation induced a significant increase in leukocyte adhesion that was dose dependent . following irradiation , P selectin deficient mice did not show any increase in leukocyte rolling but did demonstrate a response in leukocyte adhesion similar to that of the wild type mice . radiation induced dose dependent histological inflammatory damage that did not differ between P selectin deficient and wild type mice . We conclude that P selectin is up regulated following irradiation and is a key molecular determinant of leukocyte rolling but not leukocyte adhesion in this inflammatory condition . therefore , isolated neutralization of this adhesion molecule is not an effective means …

Inflammation and endothelial dysfunction are associated with retinopathy : the hoorn study. (2005 Jul)
inflammation and endothelial dysfunction are associated with retinopathy : the hoorn study . AIMS / hypothesis : The exact pathogenesis of retinopathy in diabetic and non diabetic individuals is incompletely understood , but may involve chronic low grade inflammation and dysfunction of the vascular endothelium . The aim of this study was to investigate the association of inflammation and endothelial dysfunction with prevalent retinopathy in individuals with and without type 2 diabetes . methods : As part of a population based cohort study , 625 individuals aged 50 74 years , stratified according to age , sex and glucose tolerance status , underwent an extensive physical examination . retinopathy was assessed by an ophthalmological examination , including funduscopy and two field 45 degrees fundus photography with mydriasis in both eyes . levels of C reactive protein ( CRP ) , soluble intercellular adhesion molecule 1 ( sicam 1 ) , von willebrand factor , and soluble vascular adhesion molecule 1 ( svcam 1 ) were assessed , together with the urinary albumin : creatinine ratio , and the results were combined to obtain summarising z scores for inflammation and endothelial dysfunction . results : The prevalence of retinopathy was positively associated with tertiles of CRP and sicam 1 . When compared with the lowest tertile , the highest tertile of the inflammatory z score was associated with retinopathy in all subjects ( odds ratio OR 2 . 2 , 95 CI 1 . 2 4 . 1 , adjusted for …

Blocking endothelial adhesion molecules : a potential therapeutic strategy to combat atherogenesis. (2004 Sep)
blocking endothelial adhesion molecules : a potential therapeutic strategy to combat atherogenesis . purpose OF review : This review provides a concise update of the involvement of endothelial adhesion molecules in atherogenesis , an overview of current advances in the development of adhesion molecule blocking agents , as well as an insight into the potential of these molecules in cardiovascular therapy . recent findings : As endothelial adhesion molecules are deemed to play an important role in the development and progression of atherosclerotic lesions , they are interesting targets for therapeutic intervention in this process . In particular , P selectin and vascular cell adhesion molecule 1 are widely considered to hold promise in this regard . current research efforts centre on the design of agents that directly block the interaction of the receptor with its ligand ( e . g . soluble P selectin glycoprotein ligand 1 , blocking antibodies , EWVD based peptides ) or that interfere with their synthesis ( e . g . antisense oligonucleotides ) or their regulatory control by nuclear factor kappa B or peroxisome proliferator activated receptor gamma . furthermore , adhesion molecules have been exploited as a target for the specific delivery of drug carriers ( e . g . biodegradable particles with entrapped dexamethasone ) or therapeutic compounds ( e . g . dexamethasone ) to the plaque . All approaches have been shown to be effective in blocking adhesion molecule function in in vitro studies and in vivo models for …

Endothelial leukocyte adhesion molecules. (1993 May)
endothelial leukocyte adhesion molecules . One decade ago , vascular endothelium was commonly considered a non stick lining of blood vessels that functioned only to prevent blood coagulation and to separate the vascular space from tissues . By comparison to many other cell types , endothelial cells were thought to be less active , less complex , and less interesting . since that time , research concerning the endothelium has expanded dramatically and produced a new image of the vascular lining as an active participant in a wide variety of pathophysiological processes , including inflammation and immunity . nowhere has the excitement been more intense than in the study of the molecular mechanisms of leukocyte adhesion to endothelium . recent efforts resulted in the identification , characterization , and cloning of multiple endothelial cell surface glycoproteins that support adhesion through an interaction with specific ligands ( or counter receptors ) on leukocytes . The selectins , two of which are found on endothelium and one on leukocytes , support adhesion through the recognition of carbohydrates . endothelial members of the immunoglobulin superfamily including ICAM 1 and VCAM 1 / incam 110 bind to leukocyte cell surface integrins . In various combinations , these and other molecules support leukocyte adhesion to the vessel wall and extravasation , key steps in our response to infection and tissue injury .

Circulating soluble adhesion molecules in patients with giant cell arteritis. (1999 Jun)
circulating soluble adhesion molecules in patients with giant cell arteritis . correlation between soluble intercellular adhesion molecule 1 ( sicam 1 ) concentrations and disease activity . objective : To evaluate whether changes in concentrations of circulating adhesion molecules are related to disease activity in patients with giant cell arteritis ( GCA ) . methods : A sandwich elisa was used to measure soluble intercellular adhesion molecule 1 ( sicam 1 ) , sicam 3 , vascular cell adhesion molecule 1 ( svcam 1 ) , E selectin ( sE selectin ) , and L selectin ( sL selectin ) in serum and plasma samples from patients with GCA . A cross sectional study was performed on 64 GCA patients at different activity stages and on 35 age and sex matched healthy donors . thirteen of these patients were evaluated at the time of diagnosis and serially during follow up . results : At the time of diagnosis , sicam 1 concentrations were significantly higher in active GCA patients than in controls ( mean ( SD ) 360 . 55 ( 129 . 78 ) ng / ml versus 243 . 25 ( 47 . 43 ) ng / ml , p 0 . 001 ) . In contrast , sicam 3 , svcam 1 , sE selectin , and sL selectin values did not differ from those obtained in normal donors . With corticosteroid administration , a decrease in sicam 1 concentrations was observed , reaching normal values when …

Free heme toxicity and its detoxification systems in human. (2005 May)
Free heme toxicity and its detoxification systems in human . severe hemolysis or myolysis occurring during pathological states , such as sickle cell disease , ischemia reperfusion , and malaria results in high levels of free heme , causing undesirable toxicity leading to organ , tissue , and cellular injury . Free heme catalyzes the oxidation , covalent cross linking and aggregate formation of protein and its degradation to small peptides . It also catalyzes the formation of cytotoxic lipid peroxide via lipid peroxidation and damages DNA through oxidative stress . Heme being a lipophilic molecule intercalates in the membrane and impairs lipid bilayers and organelles , such as mitochondria and nuclei , and destabilizes the cytoskeleton . Heme is a potent hemolytic agent and alters the conformation of cytoskeletal protein in red cells . Free heme causes endothelial cell injury , leading to vascular inflammatory disorders and stimulates the expression of intracellular adhesion molecules . Heme acts as a pro inflammatory molecule and heme induced inflammation is involved in the pathology of diverse conditions ; such as renal failure , arteriosclerosis , and complications after artificial blood transfusion , peritoneal endometriosis , and heart transplant failure . Heme offers severe toxic effects to kidney , liver , central nervous system and cardiac tissue . although heme oxygenase is primarily responsible to detoxify free heme but other extra heme oxygenase systems also play a significant role to detoxify heme . A brief account of free heme toxicity and its detoxification …

Leucocyte endothelial cell adhesion in a model of intestinal inflammation. (1995 Dec)
leucocyte endothelial cell adhesion in a model of intestinal inflammation . leucocyte endothelial cell adhesion is modulated by a variety of adhesion glycoprotein expressed on the surface of leucocytes and endothelial cells . although in vitro studies show that these adhesion molecules mediate the decrease in leucocyte rolling velocity and the increase in leucocyte adherence and emigration associated with inflammation , there are few in vivo data to support this hypothesis . The aim of this study was to assess the role of leucocyte ( cd11b / CD18 ) and endothelial cell ( P and E selectin ) adhesion molecules in mediating the leucocyte endothelial cell adhesion elicited in rat mesenteric venules during a model of longlasting intestinal inflammation . indomethacin was injected 48 and 24 hours before the experiment . The mesenteric microcirculation was observed by intravital microscopy in animals treated with monoclonal antibodies ( MAb ) directed against either P selectin , E selectin , or cd11b / CD18 . leucocyte rolling velocity , and the number of adherent and emigrated leucocytes as well as vessel diameter and erythrocyte velocity were monitored in roughly 30 micron diameter postcapillary venules . indomethacin treatment resulted in mucosal ulceration and granulocyte infiltration , and a corresponding inflammatory response in the mesentery , which was characterised by an increase in the number of adherent ( eightfold ) and emigrated ( sixfold ) leucocytes and a reduction ( 80 ) in leucocyte rolling velocity . The indomethacin induced leucocyte endothelial cell adhesion in …